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Important reminders
- Do NOT use bleach or any disinfectant with high chlorine content.
- Use sterile, distilled, or demineralized water.
- Avoid spraying cleaner on the CO₂ sensor.
- Do not use powdered gloves for tissue cultures.
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Maintenance schedule
| Action |
Frequency |
| Check water level in humidity pan, 1/2" from top |
Weekly |
| Change HEPA and gas filters |
6 months |
| Inspect door latch hinges and door gasket seal |
Yearly |
| Verify and document CO₂, humidity, temp calibration |
Yearly |
| Replenish rust inhibitor in water jacket |
2 years |
| Perform complete decontamination procedure |
As needed |
Filling the humidity pan
- Use sterilized, distilled, demineralized or deionized water. Water must be safe with stainless steel — no high purity, ultra pure or milli-Q water. The pan is autoclavable.
- Fill the pan to within 1/2 inch of the top with sterile, distilled water. The pan requires approximately 3 L of water to fill to within 1/2 inch of the top.
Calibration
See "Section 2: Calibration" in the manual.
Cleaning and decontamination
No bleach or any disinfectant with high chlorine content. See "Section 5: Routine Maintenance" in the manual for thorough cleaning instructions.
Changing HEPA and gas filters
See "Sections 5–6" in the manual for instructions. An alarm can also be set to alert personnel when replacements need to occur (Section 5-6).
Rust inhibitor replenishment
- Mix 1 bag or bottle of rust inhibitor with a gallon of distilled, demineralized, or deionized water.
- Rust inhibitor (0.5 lb) bag — Part # 1900100
- Rust inhibitor (800 mL) bottle — Part # 1900101
- Drain a gallon of water from the jacket and replace it with the rust inhibitor mixture.