Modified McMaster's technique for fecal egg counting.

Materials

• Wooden tongue depressors
• Sheather's sugar solution (recipe below)
• Tea strainers
• Graduated cylinder
• Glass beakers
• Transfer pipettes
• McMaster slides
• Small weigh boats

Equipment

• Microscope
• Scale

Protocol

1. Weigh out 2 grams of feces per sample.
2. Measure out 25 mL of Sheather's sugar solution using a graduated cylinder.
3. Place a tea strainer on top of a 100 mL beaker and add the feces to the strainer.
4. Pour the sugar solution incrementally over the feces and muddle or squeeze the feces through the strainer using a tongue depressor.
5. Once as much of the feces as possible has passed through the strainer, add a stir bar and allow the solution to mix thoroughly.
6. While the solution is stirring, draw up the suspension in a transfer pipette and transfer it to both chambers of a McMaster slide, ensuring no bubbles are in the grid region.