<aside> ๐๏ธ
Standard values for the metadata .csv files that accompany an experiment. Keeping these consistent is what lets replicates be combined across shipments, plates and researchers.
</aside>
<aside> ๐ก
You don't have to use every .csv for every metadata folder, and you can add your own .csv if you need to cover a parameter not listed here.
</aside>
| File | Captures |
|---|---|
treatment.csv |
Compound or control in the well |
conc.csv |
Concentration of whatever is in treatment.csv |
species.csv |
Species name |
strains.csv |
Strain within a species |
stages.csv |
Life stage |
celline.csv |
Mammalian cell line |
timepoint.csv |
Hours relative to treatment |
date.csv |
Experiment start or shipment date |
exp.csv |
Experiment or library name |
batch.csv |
Unique identifier for a group of worms |
temperature.csv |
Incubation temperature |
Common anthelmintics โ IVM, DEC, AZS (albendazole sulfoxide), ABZ (albendazole), EMO, LEV, PZQ, IDA, TBZ (thiabendazole), TCBZ (triclabendazole)
Other anthelmintics โ all lowercase, spelled out
Amines โ DOP, SER, HIS, ACH, TYR, OCT, NOR, EPI
Cholinergic โ ATR, NIC, CAR, OXO
Controls โ DMSO, HK, NaAz, Water, None
Salts โ nacl, kpo4
Anything else โ write out fully lowercase in the metadata, then make display labels in code.
<aside> โ
Use underscores when a well has more than one treatment โ e.g. nacl_kpo4.
</aside>
<aside> โ
Check this. The source doc lists the controls as "DMSO, HK, NaAz, Water, None (?)" โ the question mark suggests None was never confirmed as a valid value. Decide whether it stays.
</aside>
Concentration of whatever sits in the treatment.csv cell.
#unit with no spacepM, uM (or ยตM), nM, mM, pg/ml, ng/ml, ug/ml, mg/ml#p โ e.g. 1% DMSO is 1p