A. Staining microfilariae after blood filtration (qualitative)

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Follow the Microfilariae Purification protocol to filter microfilariae from blood.

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Materials

• Glass slides
• Giemsa stain (Modified Solution, Fluka Analytical 48900)
• Methanol (Fisher BP1105 4)

Protocol

1. After filtration, place the filter on a glass slide.
2. Fix dried mf on the filter in 100% methanol for 30–60 seconds.
3. Wash lightly with H₂O and let air dry.
4. Stain with diluted Giemsa (1:20) for 20 minutes. Store the stain at room temperature.
5. Wash with H₂O and let air dry.

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Protocol adapted from the CDC Bench Aid for Brugia spp. and from Medeiros et al. 2018.

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B. Loop-mediated isothermal amplification (LAMP) diagnostic

Materials for DNA extraction from whole blood

• Quick-DNA 96 plus kit (Zymo D4071)
• Disposable lab coats
• Sterile H₂O
• Plate foil cover (Zymo C2007-4)

DNA extraction protocol