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Zamanian Lab Protocols
Bench protocols that are fairly stable and occasionally edited.
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Mosquito-Stage Parasite Protocols
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Numbers that don't add up — flagged in place on each page, all need someone to confirm:
Single Worm Genotyping gives primer final concentration as 0.1 μM where the volumes give 1 μM. Modified McMaster's justifies its ×100 EPG multiplier with 60 mL when the protocol specifies 25 mL. Parasite Culture L3 media sums to 90 mL of a stated 100 mL and lists stocks in mg/μL. D. immitis molt media sums to 102 mL of 100 mL and states Pen-Strep at 10 U/mL where the maths gives 100. General Lab Recipes TE buffer sums to 504 mL of 500 mL. Mosquito Blood Feeding leaves the B. malayi mf concentration blank. Plate Pouring NGM is ambiguous about whether NGMA agarose replaces or adds to the agar.
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Incomplete or contradictory content: five unfinished entries in the Task List; empty Materials sections in Pharyngeal Pumping and EPG Recordings; two competing blood-filtration protocols in Microfilariae Purification with an ambiguous cross-reference; Larval Development Assay ends with no readout step; L3 Extraction calls for an undefined "G" component; Cryosectioning points at two protocols that don't exist; the Confocal shutdown sequence contradicts itself; Peggy's freezing solution mixes incompatible units; S Medium lists a duplicate phosphate buffer and omits the potassium citrate its own recipe says belongs there.
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Five protocols were never in the original index and are flagged as unlisted on their group pages: Partial Digest, Plasmid and Amplicon Sequencing, an alternate dsRNA Synthesis, Larval Development Assay, and HB101 Stock Generation.
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Step numbering was repaired in 13 protocols where the source skipped, duplicated or self-referenced steps. Each carries a note saying what was changed, so nothing is silently different from the original.
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