Multivariate phenotyping of adult filarial parasites.

Materials

• RPMI-1640 (Sigma R8758)
• Pen-Strep 10,000 U/mL (Thermo 15140122)
• Gentamicin 0.4 mg/μL (Sigma G1272-100ML)
• FBS (Thermo 16000044)
• Drug solutions
• 24-well plates (Corning 3524)
• 96-well deep well plates (VWR 76210-518)
• 96-well plates used for ImageXpress (USA Scientific 5665-5180Q)
• 1.5 mL tubes
• TempPlate Sealing Film (USA Scientific 2921-0010)
• Tools for worm handling (probes, forceps)

Equipment

• 37°C CO₂ incubator
• WormViz
• Eppendorf microcentrifuge
• Sterile BSC
• Eppendorf M4 repeater pipette
• ImageXpress Nano (for quantifying microfilaria release)